Cargando…

Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing

Anti-CRISPR (Acr) proteins are encoded by many mobile genetic elements (MGEs) such as phages and plasmids to combat CRISPR–Cas adaptive immune systems employed by prokaryotes, which provide powerful tools for CRISPR–Cas-based applications. Here, we discovered nine distinct type II-A anti-CRISPR (Acr...

Descripción completa

Detalles Bibliográficos
Autores principales:	Song, Guoxu, Zhang, Fei, Tian, Chunhong, Gao, Xing, Zhu, Xiaoxiao, Fan, Dongdong, Tian, Yong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934645/ https://www.ncbi.nlm.nih.gov/pubmed/35188577 http://dx.doi.org/10.1093/nar/gkac099

Ejemplares similares

Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells
por: Tao, Rui, et al.
Publicado: (2022)

Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA
por: Paul, Bijoya, et al.
Publicado: (2021)

Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants
por: Murugan, Karthik, et al.
Publicado: (2021)

Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity
por: Hu, Ziying, et al.
Publicado: (2021)

Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system
por: Sokolowski, Richard D., et al.
Publicado: (2014)

Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9
por: Okada, Keita, et al.
Publicado: (2022)

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems
por: Fonfara, Ines, et al.
Publicado: (2014)

In-depth assessment of the PAM compatibility and editing activities of Cas9 variants
por: Zhang, Weiwei, et al.
Publicado: (2021)

DNA targeting by Clostridium cellulolyticum CRISPR–Cas9 Type II-C system
por: Fedorova, Iana, et al.
Publicado: (2020)

Cas1–Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation
por: Killelea, Tom, et al.
Publicado: (2023)

Enhanced Cas12a editing in mammalian cells and zebrafish
por: Liu, Pengpeng, et al.
Publicado: (2019)

Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
por: Mekler, Vladimir, et al.
Publicado: (2016)

DNA binding specificities of Escherichia coli Cas1–Cas2 integrase drive its recruitment at the CRISPR locus
por: Moch, Clara, et al.
Publicado: (2017)

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage
por: Mullally, Grace, et al.
Publicado: (2020)

Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system
por: Gleditzsch, Daniel, et al.
Publicado: (2016)

CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity
por: Marino, Nicole D, et al.
Publicado: (2022)

Active site plasticity enables metal-dependent tuning of Cas5d nuclease activity in CRISPR-Cas type I-C system
por: Punetha, Ankita, et al.
Publicado: (2014)

In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex
por: Plagens, André, et al.
Publicado: (2014)

Interference activity of a minimal Type I CRISPR–Cas system from Shewanella putrefaciens
por: Dwarakanath, Srivatsa, et al.
Publicado: (2015)

Different modes of spacer acquisition by the Staphylococcus epidermidis type III-A CRISPR-Cas system
por: Aviram, Naama, et al.
Publicado: (2022)

Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases
por: Molina, Rafael, et al.
Publicado: (2022)

Casposon integration shows strong target site preference and recapitulates protospacer integration by CRISPR-Cas systems
por: Béguin, Pierre, et al.
Publicado: (2016)

A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction
por: Han, Wenyuan, et al.
Publicado: (2017)

Enhancement of target specificity of CRISPR–Cas12a by using a chimeric DNA–RNA guide
por: Kim, Hanseop, et al.
Publicado: (2020)

A DNA unwinding equilibrium serves as a checkpoint for CRISPR-Cas12a target discrimination
por: Singh, Jaideep, et al.
Publicado: (2023)

Asymmetric positioning of Cas1–2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system
por: Yoganand, K.N.R., et al.
Publicado: (2017)

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
por: Karvelis, Tautvydas, et al.
Publicado: (2020)

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR–Cas effector complexes
por: Vlot, Marnix, et al.
Publicado: (2018)

PpCas9 from Pasteurella pneumotropica — a compact Type II-C Cas9 ortholog active in human cells
por: Fedorova, Iana, et al.
Publicado: (2020)

Progressive engineering of a homing endonuclease genome editing reagent for the murine X-linked immunodeficiency locus
por: Wang, Yupeng, et al.
Publicado: (2014)

Cmr1 enables efficient RNA and DNA interference of a III-B CRISPR–Cas system by binding to target RNA and crRNA
por: Li, Yingjun, et al.
Publicado: (2017)

A type III-A CRISPR–Cas system mediates co-transcriptional DNA cleavage at the transcriptional bubbles in close proximity to active effectors
por: Lin, Jinzhong, et al.
Publicado: (2021)

A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration
por: Wu, You, et al.
Publicado: (2022)

Unidirectional trans-cleaving behavior of CRISPR-Cas12a unlocks for an ultrasensitive assay using hybrid DNA reporters containing a 3′ toehold
por: Mohammad, Noor, et al.
Publicado: (2023)

CasDinG is a 5′-3′ dsDNA and RNA/DNA helicase with three accessory domains essential for type IV CRISPR immunity
por: Domgaard, Hannah, et al.
Publicado: (2023)

Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo
por: Fu, Becky X.H., et al.
Publicado: (2016)

The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity
por: Lemak, Sofia, et al.
Publicado: (2014)

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
por: Kato-Inui, Tomoko, et al.
Publicado: (2018)

Probing the stability of the SpCas9–DNA complex after cleavage
por: Aldag, Pierre, et al.
Publicado: (2021)

Guide-specific loss of efficiency and off-target reduction with Cas9 variants
por: Zhang, Liang, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ao5bt71ue8o2nshp4d095t4r2v