Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

Specific sequence features of the protospacer and protospacer-adjacent motif (PAM) are critical for efficient cleavage by CRISPR-Cas9, but current knowledge is largely derived from single-guide RNA (sgRNA) systems assessed in cultured cells. In this study, we sought to determine gRNA sequence featur...

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Detalles Bibliográficos
Autores principales: Okada, Keita, Aoki, Kanae, Tabei, Teruyuki, Sugio, Kota, Imai, Katsunori, Bonkohara, Yuki, Kamachi, Yusuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934663/
https://www.ncbi.nlm.nih.gov/pubmed/35166844
http://dx.doi.org/10.1093/nar/gkac100

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934663/
https://www.ncbi.nlm.nih.gov/pubmed/35166844
http://dx.doi.org/10.1093/nar/gkac100

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