Engineered Sleeping Beauty transposase redirects transposon integration away from genes

The Sleeping Beauty (SB) transposon system is a popular tool for genome engineering, but random integration into the genome carries a certain genotoxic risk in therapeutic applications. Here we investigate the role of amino acids H187, P247 and K248 in target site selection of the SB transposase. St...

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Detalles Bibliográficos
Autores principales: Miskey, Csaba, Kesselring, Lisa, Querques, Irma, Abrusán, György, Barabas, Orsolya, Ivics, Zoltán
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934666/
https://www.ncbi.nlm.nih.gov/pubmed/35188569
http://dx.doi.org/10.1093/nar/gkac092

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934666/
https://www.ncbi.nlm.nih.gov/pubmed/35188569
http://dx.doi.org/10.1093/nar/gkac092

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