Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules

In-situ oral delivery of therapeutic antibodies, like monoclonal antibody, for chronic inflammation treatment is the most convenient approach compared with other administration routes. Moreover, the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or a...

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Autores principales: Li, Bo, Li, Xin, Chu, Xiaodong, Lou, Pengcheng, Yuan, Yin, Zhuge, Aoxiang, Zhu, Xueling, Shen, Yangfan, Pan, Jinghua, Zhang, Liyuan, Li, Lanjuan, Wu, Zhongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935091/
https://www.ncbi.nlm.nih.gov/pubmed/35356818
http://dx.doi.org/10.1016/j.bioactmat.2021.12.022
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author Li, Bo
Li, Xin
Chu, Xiaodong
Lou, Pengcheng
Yuan, Yin
Zhuge, Aoxiang
Zhu, Xueling
Shen, Yangfan
Pan, Jinghua
Zhang, Liyuan
Li, Lanjuan
Wu, Zhongwen
author_facet Li, Bo
Li, Xin
Chu, Xiaodong
Lou, Pengcheng
Yuan, Yin
Zhuge, Aoxiang
Zhu, Xueling
Shen, Yangfan
Pan, Jinghua
Zhang, Liyuan
Li, Lanjuan
Wu, Zhongwen
author_sort Li, Bo
collection PubMed
description In-situ oral delivery of therapeutic antibodies, like monoclonal antibody, for chronic inflammation treatment is the most convenient approach compared with other administration routes. Moreover, the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or antagonistic effect of gut microbiota to colonic inflammation. However, the antibody activity would be significantly affected while transferring through the gastrointestinal tract due to hostile conditions. Moreover, these antibodies have short serum half-lives, thus, require to be frequently administered with high doses to be effective, leading to low patient tolerance. Here, we develop a strategy utilizing thin shell hydrogel microcapsule fabricated by microfluidic technique as the oral delivering carrier. By encapsulating antibodies in these microcapsules, antibodies survive in the hostile gastrointestinal environment and rapidly release into the small intestine through oral administration route, achieving the same therapeutic effect as the intravenous injection evaluated by a colonic inflammation disease model. Moreover, the abundance of some intestinal microorganisms as the indication of the improvement of inflammation has remarkably altered after in-situ antibody-laden microcapsules delivery, implying the restoration of micro-ecology of the intestine. These findings prove our microcapsules are exploited as an efficient oral delivery agent for antibodies with programmable function in clinical application.
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spelling pubmed-89350912022-03-29 Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules Li, Bo Li, Xin Chu, Xiaodong Lou, Pengcheng Yuan, Yin Zhuge, Aoxiang Zhu, Xueling Shen, Yangfan Pan, Jinghua Zhang, Liyuan Li, Lanjuan Wu, Zhongwen Bioact Mater Article In-situ oral delivery of therapeutic antibodies, like monoclonal antibody, for chronic inflammation treatment is the most convenient approach compared with other administration routes. Moreover, the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or antagonistic effect of gut microbiota to colonic inflammation. However, the antibody activity would be significantly affected while transferring through the gastrointestinal tract due to hostile conditions. Moreover, these antibodies have short serum half-lives, thus, require to be frequently administered with high doses to be effective, leading to low patient tolerance. Here, we develop a strategy utilizing thin shell hydrogel microcapsule fabricated by microfluidic technique as the oral delivering carrier. By encapsulating antibodies in these microcapsules, antibodies survive in the hostile gastrointestinal environment and rapidly release into the small intestine through oral administration route, achieving the same therapeutic effect as the intravenous injection evaluated by a colonic inflammation disease model. Moreover, the abundance of some intestinal microorganisms as the indication of the improvement of inflammation has remarkably altered after in-situ antibody-laden microcapsules delivery, implying the restoration of micro-ecology of the intestine. These findings prove our microcapsules are exploited as an efficient oral delivery agent for antibodies with programmable function in clinical application. KeAi Publishing 2021-12-25 /pmc/articles/PMC8935091/ /pubmed/35356818 http://dx.doi.org/10.1016/j.bioactmat.2021.12.022 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Li, Bo
Li, Xin
Chu, Xiaodong
Lou, Pengcheng
Yuan, Yin
Zhuge, Aoxiang
Zhu, Xueling
Shen, Yangfan
Pan, Jinghua
Zhang, Liyuan
Li, Lanjuan
Wu, Zhongwen
Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules
title Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules
title_full Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules
title_fullStr Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules
title_full_unstemmed Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules
title_short Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules
title_sort micro-ecology restoration of colonic inflammation by in-situ oral delivery of antibody-laden hydrogel microcapsules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935091/
https://www.ncbi.nlm.nih.gov/pubmed/35356818
http://dx.doi.org/10.1016/j.bioactmat.2021.12.022
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