Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules
In-situ oral delivery of therapeutic antibodies, like monoclonal antibody, for chronic inflammation treatment is the most convenient approach compared with other administration routes. Moreover, the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or a...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935091/ https://www.ncbi.nlm.nih.gov/pubmed/35356818 http://dx.doi.org/10.1016/j.bioactmat.2021.12.022 |
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author | Li, Bo Li, Xin Chu, Xiaodong Lou, Pengcheng Yuan, Yin Zhuge, Aoxiang Zhu, Xueling Shen, Yangfan Pan, Jinghua Zhang, Liyuan Li, Lanjuan Wu, Zhongwen |
author_facet | Li, Bo Li, Xin Chu, Xiaodong Lou, Pengcheng Yuan, Yin Zhuge, Aoxiang Zhu, Xueling Shen, Yangfan Pan, Jinghua Zhang, Liyuan Li, Lanjuan Wu, Zhongwen |
author_sort | Li, Bo |
collection | PubMed |
description | In-situ oral delivery of therapeutic antibodies, like monoclonal antibody, for chronic inflammation treatment is the most convenient approach compared with other administration routes. Moreover, the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or antagonistic effect of gut microbiota to colonic inflammation. However, the antibody activity would be significantly affected while transferring through the gastrointestinal tract due to hostile conditions. Moreover, these antibodies have short serum half-lives, thus, require to be frequently administered with high doses to be effective, leading to low patient tolerance. Here, we develop a strategy utilizing thin shell hydrogel microcapsule fabricated by microfluidic technique as the oral delivering carrier. By encapsulating antibodies in these microcapsules, antibodies survive in the hostile gastrointestinal environment and rapidly release into the small intestine through oral administration route, achieving the same therapeutic effect as the intravenous injection evaluated by a colonic inflammation disease model. Moreover, the abundance of some intestinal microorganisms as the indication of the improvement of inflammation has remarkably altered after in-situ antibody-laden microcapsules delivery, implying the restoration of micro-ecology of the intestine. These findings prove our microcapsules are exploited as an efficient oral delivery agent for antibodies with programmable function in clinical application. |
format | Online Article Text |
id | pubmed-8935091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-89350912022-03-29 Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules Li, Bo Li, Xin Chu, Xiaodong Lou, Pengcheng Yuan, Yin Zhuge, Aoxiang Zhu, Xueling Shen, Yangfan Pan, Jinghua Zhang, Liyuan Li, Lanjuan Wu, Zhongwen Bioact Mater Article In-situ oral delivery of therapeutic antibodies, like monoclonal antibody, for chronic inflammation treatment is the most convenient approach compared with other administration routes. Moreover, the abundant links between the gut microbiota and colonic inflammation indicate that the synergistic or antagonistic effect of gut microbiota to colonic inflammation. However, the antibody activity would be significantly affected while transferring through the gastrointestinal tract due to hostile conditions. Moreover, these antibodies have short serum half-lives, thus, require to be frequently administered with high doses to be effective, leading to low patient tolerance. Here, we develop a strategy utilizing thin shell hydrogel microcapsule fabricated by microfluidic technique as the oral delivering carrier. By encapsulating antibodies in these microcapsules, antibodies survive in the hostile gastrointestinal environment and rapidly release into the small intestine through oral administration route, achieving the same therapeutic effect as the intravenous injection evaluated by a colonic inflammation disease model. Moreover, the abundance of some intestinal microorganisms as the indication of the improvement of inflammation has remarkably altered after in-situ antibody-laden microcapsules delivery, implying the restoration of micro-ecology of the intestine. These findings prove our microcapsules are exploited as an efficient oral delivery agent for antibodies with programmable function in clinical application. KeAi Publishing 2021-12-25 /pmc/articles/PMC8935091/ /pubmed/35356818 http://dx.doi.org/10.1016/j.bioactmat.2021.12.022 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Li, Bo Li, Xin Chu, Xiaodong Lou, Pengcheng Yuan, Yin Zhuge, Aoxiang Zhu, Xueling Shen, Yangfan Pan, Jinghua Zhang, Liyuan Li, Lanjuan Wu, Zhongwen Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules |
title | Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules |
title_full | Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules |
title_fullStr | Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules |
title_full_unstemmed | Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules |
title_short | Micro-ecology restoration of colonic inflammation by in-Situ oral delivery of antibody-laden hydrogel microcapsules |
title_sort | micro-ecology restoration of colonic inflammation by in-situ oral delivery of antibody-laden hydrogel microcapsules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935091/ https://www.ncbi.nlm.nih.gov/pubmed/35356818 http://dx.doi.org/10.1016/j.bioactmat.2021.12.022 |
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