Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes

SLC6A20A is a proline and glycine transporter known to regulate glycine homeostasis and NMDA receptor (NMDAR) function in the brain. A previous study found increases in ambient glycine levels and NMDA receptor-mediated synaptic transmission in the brains of Slc6a20a-haploinsufficient mice, but it re...

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Autores principales: Kim, Junhyung, Roh, Junyeop Daniel, Kim, Seongbin, Kang, Hyojin, Bae, Mihyun, Kim, Eunjoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936588/
https://www.ncbi.nlm.nih.gov/pubmed/35321029
http://dx.doi.org/10.3389/fnmol.2022.857820
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author Kim, Junhyung
Roh, Junyeop Daniel
Kim, Seongbin
Kang, Hyojin
Bae, Mihyun
Kim, Eunjoon
author_facet Kim, Junhyung
Roh, Junyeop Daniel
Kim, Seongbin
Kang, Hyojin
Bae, Mihyun
Kim, Eunjoon
author_sort Kim, Junhyung
collection PubMed
description SLC6A20A is a proline and glycine transporter known to regulate glycine homeostasis and NMDA receptor (NMDAR) function in the brain. A previous study found increases in ambient glycine levels and NMDA receptor-mediated synaptic transmission in the brains of Slc6a20a-haploinsufficient mice, but it remained unknown whether Slc6a20a deficiency leads to disease-related behavioral deficits in mice. Here, we report that Slc6a20a heterozygous and homozygous mutant mice display differential behavioral phenotypes in locomotor, repetitive behavioral, and spatial and fear memory domains. In addition, these mice show differential transcriptomic changes in synapse, ribosome, mitochondria, autism, epilepsy, and neuron-related genes. These results suggest that heterozygous and homozygous Slc6a20a deletions in mice lead to differential changes in behaviors and transcriptomes.
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spelling pubmed-89365882022-03-22 Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes Kim, Junhyung Roh, Junyeop Daniel Kim, Seongbin Kang, Hyojin Bae, Mihyun Kim, Eunjoon Front Mol Neurosci Molecular Neuroscience SLC6A20A is a proline and glycine transporter known to regulate glycine homeostasis and NMDA receptor (NMDAR) function in the brain. A previous study found increases in ambient glycine levels and NMDA receptor-mediated synaptic transmission in the brains of Slc6a20a-haploinsufficient mice, but it remained unknown whether Slc6a20a deficiency leads to disease-related behavioral deficits in mice. Here, we report that Slc6a20a heterozygous and homozygous mutant mice display differential behavioral phenotypes in locomotor, repetitive behavioral, and spatial and fear memory domains. In addition, these mice show differential transcriptomic changes in synapse, ribosome, mitochondria, autism, epilepsy, and neuron-related genes. These results suggest that heterozygous and homozygous Slc6a20a deletions in mice lead to differential changes in behaviors and transcriptomes. Frontiers Media S.A. 2022-03-07 /pmc/articles/PMC8936588/ /pubmed/35321029 http://dx.doi.org/10.3389/fnmol.2022.857820 Text en Copyright © 2022 Kim, Roh, Kim, Kang, Bae and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Kim, Junhyung
Roh, Junyeop Daniel
Kim, Seongbin
Kang, Hyojin
Bae, Mihyun
Kim, Eunjoon
Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes
title Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes
title_full Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes
title_fullStr Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes
title_full_unstemmed Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes
title_short Slc6a20a Heterozygous and Homozygous Mutant Mice Display Differential Behavioral and Transcriptomic Changes
title_sort slc6a20a heterozygous and homozygous mutant mice display differential behavioral and transcriptomic changes
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936588/
https://www.ncbi.nlm.nih.gov/pubmed/35321029
http://dx.doi.org/10.3389/fnmol.2022.857820
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