Understanding desaturation/hydroxylation activity of castor stearoyl Δ(9)-Desaturase through rational mutagenesis

A recently proposed reaction mechanism of soluble Δ(9) desaturase (Δ(9)D) allowed us to identify auxiliary residues His203, Asp101, Thr206 and Cys222 localized near the di-iron active site that are supposedly involved in the proton transfer (PT) to and from the active site. The PT, along with the el...

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Detalles Bibliográficos
Autores principales: Tupec, Michal, Culka, Martin, Machara, Aleš, Macháček, Stanislav, Bím, Daniel, Svatoš, Aleš, Rulíšek, Lubomír, Pichová, Iva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8940945/
https://www.ncbi.nlm.nih.gov/pubmed/35386101
http://dx.doi.org/10.1016/j.csbj.2022.03.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8940945/
https://www.ncbi.nlm.nih.gov/pubmed/35386101
http://dx.doi.org/10.1016/j.csbj.2022.03.010

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