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Understanding desaturation/hydroxylation activity of castor stearoyl Δ(9)-Desaturase through rational mutagenesis
A recently proposed reaction mechanism of soluble Δ(9) desaturase (Δ(9)D) allowed us to identify auxiliary residues His203, Asp101, Thr206 and Cys222 localized near the di-iron active site that are supposedly involved in the proton transfer (PT) to and from the active site. The PT, along with the el...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Research Network of Computational and Structural Biotechnology
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8940945/ https://www.ncbi.nlm.nih.gov/pubmed/35386101 http://dx.doi.org/10.1016/j.csbj.2022.03.010 |