High-resolution melting analysis after nested PCR for the detection of SARS-CoV-2 spike protein G339D and D796Y variations

High-resolution melting (HRM) analysis was performed to detect G339D and D796Y variations in the SARS-CoV-2 Omicron variant spike protein. We employed two-step PCR consisting of the first RT–PCR and the second nested PCR to prepare the amplicon for HRM analysis. The melting temperatures (Tm) of the...

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Detalles Bibliográficos
Autores principales: Miyoshi, Hiroshi, Ichinohe, Ryu, Koshikawa, Takuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8942459/
https://www.ncbi.nlm.nih.gov/pubmed/35349821
http://dx.doi.org/10.1016/j.bbrc.2022.03.083

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8942459/
https://www.ncbi.nlm.nih.gov/pubmed/35349821
http://dx.doi.org/10.1016/j.bbrc.2022.03.083

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