ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish

SIMPLE SUMMARY: CRISPR/Cas9 enables efficient mutagenesis and generation of various knockout and knockin alleles in many species including zebrafish. However, the application of the Cas12a nuclease in zebrafish is far from ideal due to demanding experimental conditions, especially the requirements f...

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Detalles Bibliográficos
Autores principales: Han, Bingzhou, Zhang, Yage, Zhou, Yang, Zhang, Biao, Krueger, Christopher J., Bi, Xuetong, Zhu, Zuoyan, Tong, Xiangjun, Zhang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945719/
https://www.ncbi.nlm.nih.gov/pubmed/35336785
http://dx.doi.org/10.3390/biology11030411

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945719/
https://www.ncbi.nlm.nih.gov/pubmed/35336785
http://dx.doi.org/10.3390/biology11030411

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