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Impact of Molecule Concentration, Diffusion Rates and Surface Passivation on Single-Molecule Fluorescence Studies in Solution

For single-molecule studies in solution, very small concentrations of dye-labelled molecules are employed in order to achieve single-molecule sensitivity. In typical studies with confocal microscopes, often concentrations in the pico-molar regime are required. For various applications that make use...

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Detalles Bibliográficos
Autores principales: Yukhnovets, Olessya, Höfig, Henning, Bustorff, Nuno, Katranidis, Alexandros, Fitter, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946791/
https://www.ncbi.nlm.nih.gov/pubmed/35327660
http://dx.doi.org/10.3390/biom12030468