Cargando…
Impact of Molecule Concentration, Diffusion Rates and Surface Passivation on Single-Molecule Fluorescence Studies in Solution
For single-molecule studies in solution, very small concentrations of dye-labelled molecules are employed in order to achieve single-molecule sensitivity. In typical studies with confocal microscopes, often concentrations in the pico-molar regime are required. For various applications that make use...
Autores principales: | Yukhnovets, Olessya, Höfig, Henning, Bustorff, Nuno, Katranidis, Alexandros, Fitter, Jörg |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946791/ https://www.ncbi.nlm.nih.gov/pubmed/35327660 http://dx.doi.org/10.3390/biom12030468 |
Ejemplares similares
-
Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
por: Höfig, Henning, et al.
Publicado: (2019) -
The Thermodynamic Fingerprints of Ultra-Tight Nanobody–Antigen Binding Probed via Two-Color Single-Molecule Coincidence Detection
por: Schedler, Benno, et al.
Publicado: (2023) -
Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species
por: Höfig, Henning, et al.
Publicado: (2018) -
Features of Protein Unfolding Transitions and Their Relation to Domain Topology Probed by Single-Molecule FRET
por: Bustorff, Nuno, et al.
Publicado: (2023) -
A Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems
por: Kempf, Noémie, et al.
Publicado: (2017)