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Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections

Salmonella enterica is the most common foodborne pathogen worldwide. It causes two types of diseases, a self-limiting gastroenteritis and an invasive, more threatening, infection. Salmonella gastroenteritis is caused by several serotypes and is common worldwide. In contrast, invasive salmonellosis i...

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Autores principales: Dieye, Yakhya, Hull, Dawn M., Wane, Abdoul Aziz, Harden, Lyndy, Fall, Cheikh, Sambe-Ba, Bissoume, Seck, Abdoulaye, Fedorka-Cray, Paula J., Thakur, Siddhartha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947133/
https://www.ncbi.nlm.nih.gov/pubmed/35325007
http://dx.doi.org/10.1371/journal.pone.0266025
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author Dieye, Yakhya
Hull, Dawn M.
Wane, Abdoul Aziz
Harden, Lyndy
Fall, Cheikh
Sambe-Ba, Bissoume
Seck, Abdoulaye
Fedorka-Cray, Paula J.
Thakur, Siddhartha
author_facet Dieye, Yakhya
Hull, Dawn M.
Wane, Abdoul Aziz
Harden, Lyndy
Fall, Cheikh
Sambe-Ba, Bissoume
Seck, Abdoulaye
Fedorka-Cray, Paula J.
Thakur, Siddhartha
author_sort Dieye, Yakhya
collection PubMed
description Salmonella enterica is the most common foodborne pathogen worldwide. It causes two types of diseases, a self-limiting gastroenteritis and an invasive, more threatening, infection. Salmonella gastroenteritis is caused by several serotypes and is common worldwide. In contrast, invasive salmonellosis is rare in high-income countries (HIC) while frequent in low- and middle-income countries (LMIC), especially in sub-Saharan Africa (sSA). Invasive Nontyphoidal Salmonella (iNTS), corresponding to serotypes other than Typhi and Paratyphi, have emerged in sSA and pose a significant risk to public health. We conducted a whole-genome sequence (WGS) analysis of 72 strains of Salmonella isolated from diarrheic human patients and chicken meat sold in multipurpose markets in Dakar, Senegal. Antimicrobial susceptibility testing combined with WGS data analysis revealed frequent resistance to fluoroquinolones and the sulfamethoxazole-trimethoprim combination that are among the most used treatments for invasive Salmonella. In contrast, resistance to the historical first-line drugs chloramphenicol and ampicillin, and to cephalosporins was rare. Antimicrobial resistance (AMR) was lower in clinical isolates compared to chicken strains pointing to the concern posed by the excessive use of antimicrobials in farming. Phylogenetic analysis suggested possible transmission of the emerging multidrug resistant (MDR) Kentucky ST198 and serotype Schwarzengrund from chicken to human. These results stress the need for active surveillance of Salmonella and AMR in order to address invasive salmonellosis caused by nontyphoidal Salmonella strains and other important bacterial diseases in sSA.
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spelling pubmed-89471332022-03-25 Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections Dieye, Yakhya Hull, Dawn M. Wane, Abdoul Aziz Harden, Lyndy Fall, Cheikh Sambe-Ba, Bissoume Seck, Abdoulaye Fedorka-Cray, Paula J. Thakur, Siddhartha PLoS One Research Article Salmonella enterica is the most common foodborne pathogen worldwide. It causes two types of diseases, a self-limiting gastroenteritis and an invasive, more threatening, infection. Salmonella gastroenteritis is caused by several serotypes and is common worldwide. In contrast, invasive salmonellosis is rare in high-income countries (HIC) while frequent in low- and middle-income countries (LMIC), especially in sub-Saharan Africa (sSA). Invasive Nontyphoidal Salmonella (iNTS), corresponding to serotypes other than Typhi and Paratyphi, have emerged in sSA and pose a significant risk to public health. We conducted a whole-genome sequence (WGS) analysis of 72 strains of Salmonella isolated from diarrheic human patients and chicken meat sold in multipurpose markets in Dakar, Senegal. Antimicrobial susceptibility testing combined with WGS data analysis revealed frequent resistance to fluoroquinolones and the sulfamethoxazole-trimethoprim combination that are among the most used treatments for invasive Salmonella. In contrast, resistance to the historical first-line drugs chloramphenicol and ampicillin, and to cephalosporins was rare. Antimicrobial resistance (AMR) was lower in clinical isolates compared to chicken strains pointing to the concern posed by the excessive use of antimicrobials in farming. Phylogenetic analysis suggested possible transmission of the emerging multidrug resistant (MDR) Kentucky ST198 and serotype Schwarzengrund from chicken to human. These results stress the need for active surveillance of Salmonella and AMR in order to address invasive salmonellosis caused by nontyphoidal Salmonella strains and other important bacterial diseases in sSA. Public Library of Science 2022-03-24 /pmc/articles/PMC8947133/ /pubmed/35325007 http://dx.doi.org/10.1371/journal.pone.0266025 Text en © 2022 Dieye et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dieye, Yakhya
Hull, Dawn M.
Wane, Abdoul Aziz
Harden, Lyndy
Fall, Cheikh
Sambe-Ba, Bissoume
Seck, Abdoulaye
Fedorka-Cray, Paula J.
Thakur, Siddhartha
Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections
title Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections
title_full Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections
title_fullStr Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections
title_full_unstemmed Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections
title_short Genomics of human and chicken Salmonella isolates in Senegal: Broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections
title_sort genomics of human and chicken salmonella isolates in senegal: broilers as a source of antimicrobial resistance and potentially invasive nontyphoidal salmonellosis infections
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947133/
https://www.ncbi.nlm.nih.gov/pubmed/35325007
http://dx.doi.org/10.1371/journal.pone.0266025
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