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Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila

Epigenetic alterations occur during aging, but it remains unclear what epigenetic features are associated with the onset of physiological decline in animals. Nuclear lamin-B forms the filamentous meshwork underneath the nuclear envelope, providing the structural scaffold necessary for genome organiz...

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Autores principales: Lin, Wei-Qi, Ngian, Zhen-Kai, Koh, Tong-Wey, Ong, Chin-Tong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947137/
https://www.ncbi.nlm.nih.gov/pubmed/35324942
http://dx.doi.org/10.1371/journal.pone.0265223
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author Lin, Wei-Qi
Ngian, Zhen-Kai
Koh, Tong-Wey
Ong, Chin-Tong
author_facet Lin, Wei-Qi
Ngian, Zhen-Kai
Koh, Tong-Wey
Ong, Chin-Tong
author_sort Lin, Wei-Qi
collection PubMed
description Epigenetic alterations occur during aging, but it remains unclear what epigenetic features are associated with the onset of physiological decline in animals. Nuclear lamin-B forms the filamentous meshwork underneath the nuclear envelope, providing the structural scaffold necessary for genome organization and gene regulation. We found that reduced level of nuclear lamin-B protein coincides with the decline in locomotor activity and stress resistance in young adult male Drosophila. Ubiquitous lamin-B expression improves locomotor activity of the male flies at the expense of lower stress resistance and shorten lifespan. This observation suggests that tissue-specific expression of lamin-B may regulate different aspects of animal physiology during aging. To test this hypothesis, specific GAL-4 lines were used to drive the expression of lamin-B in specific neuronal populations and muscle tissues in male flies. Ectopic expression of lamin-B in the dopaminergic neurons within the protocerebral anterior medial region of the brain improves the locomotor activity of the male flies with little impact on their stress responses and lifespan. Interestingly, age-dependent decrease in the level of lamin-B protein is independent of its mRNA expression. Instead, cellular thermal shift assay showed that lamin-B and CP190 insulator protein undergo significant change in their solubility during aging. This suggests that the increased solubility of lamin-B protein may contribute to its reduced stability and degradation during aging.
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spelling pubmed-89471372022-03-25 Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila Lin, Wei-Qi Ngian, Zhen-Kai Koh, Tong-Wey Ong, Chin-Tong PLoS One Research Article Epigenetic alterations occur during aging, but it remains unclear what epigenetic features are associated with the onset of physiological decline in animals. Nuclear lamin-B forms the filamentous meshwork underneath the nuclear envelope, providing the structural scaffold necessary for genome organization and gene regulation. We found that reduced level of nuclear lamin-B protein coincides with the decline in locomotor activity and stress resistance in young adult male Drosophila. Ubiquitous lamin-B expression improves locomotor activity of the male flies at the expense of lower stress resistance and shorten lifespan. This observation suggests that tissue-specific expression of lamin-B may regulate different aspects of animal physiology during aging. To test this hypothesis, specific GAL-4 lines were used to drive the expression of lamin-B in specific neuronal populations and muscle tissues in male flies. Ectopic expression of lamin-B in the dopaminergic neurons within the protocerebral anterior medial region of the brain improves the locomotor activity of the male flies with little impact on their stress responses and lifespan. Interestingly, age-dependent decrease in the level of lamin-B protein is independent of its mRNA expression. Instead, cellular thermal shift assay showed that lamin-B and CP190 insulator protein undergo significant change in their solubility during aging. This suggests that the increased solubility of lamin-B protein may contribute to its reduced stability and degradation during aging. Public Library of Science 2022-03-24 /pmc/articles/PMC8947137/ /pubmed/35324942 http://dx.doi.org/10.1371/journal.pone.0265223 Text en © 2022 Lin et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lin, Wei-Qi
Ngian, Zhen-Kai
Koh, Tong-Wey
Ong, Chin-Tong
Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila
title Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila
title_full Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila
title_fullStr Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila
title_full_unstemmed Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila
title_short Altered stability of nuclear lamin-B marks the onset of aging in male Drosophila
title_sort altered stability of nuclear lamin-b marks the onset of aging in male drosophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947137/
https://www.ncbi.nlm.nih.gov/pubmed/35324942
http://dx.doi.org/10.1371/journal.pone.0265223
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