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Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification

Studying the entire virus replication cycle of SARS-CoV-2 is essential to identify the host factors involved and treatments to combat infection. Quantification of released virions often requires lengthy procedures, whereas quantification of viral RNA in supernatant is faster and applicable to clinic...

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Detalles Bibliográficos
Autores principales: Craig, Nicky, Fletcher, Sarah L., Daniels, Alison, Newman, Caitlin, O’Shea, Marie, Tan, Wenfang Spring, Warr, Amanda, Tait-Burkard, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949636/
https://www.ncbi.nlm.nih.gov/pubmed/35336915
http://dx.doi.org/10.3390/v14030508