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Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification
Studying the entire virus replication cycle of SARS-CoV-2 is essential to identify the host factors involved and treatments to combat infection. Quantification of released virions often requires lengthy procedures, whereas quantification of viral RNA in supernatant is faster and applicable to clinic...
Autores principales: | Craig, Nicky, Fletcher, Sarah L., Daniels, Alison, Newman, Caitlin, O’Shea, Marie, Tan, Wenfang Spring, Warr, Amanda, Tait-Burkard, Christine |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949636/ https://www.ncbi.nlm.nih.gov/pubmed/35336915 http://dx.doi.org/10.3390/v14030508 |
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