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A New 3-Ketosteroid-Δ(1)–Dehydrogenase with High Activity and Broad Substrate Scope for Efficient Transformation of Hydrocortisone at High Substrate Concentration
3-Ketosteroid-Δ(1)-dehydrogenases (KstDs [EC 1.3.99.4]) catalyze the Δ(1)-dehydrogenation of steroids and are a class of important enzymes for steroid biotransformations. In this study, nine putative kstD genes from different origins were selected and overexpressed in Escherichia coli BL21(DE3). The...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950399/ https://www.ncbi.nlm.nih.gov/pubmed/35336084 http://dx.doi.org/10.3390/microorganisms10030508 |