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A New 3-Ketosteroid-Δ(1)–Dehydrogenase with High Activity and Broad Substrate Scope for Efficient Transformation of Hydrocortisone at High Substrate Concentration

3-Ketosteroid-Δ(1)-dehydrogenases (KstDs [EC 1.3.99.4]) catalyze the Δ(1)-dehydrogenation of steroids and are a class of important enzymes for steroid biotransformations. In this study, nine putative kstD genes from different origins were selected and overexpressed in Escherichia coli BL21(DE3). The...

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Detalles Bibliográficos
Autores principales:	Wang, Yu, Zhang, Rui, Feng, Jinhui, Wu, Qiaqing, Zhu, Dunming, Ma, Yanhe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950399/ https://www.ncbi.nlm.nih.gov/pubmed/35336084 http://dx.doi.org/10.3390/microorganisms10030508

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