Obtaining Reliable RT-qPCR Results in Molecular Diagnostics—MIQE Goals and Pitfalls for Transcriptional Biomarker Discovery

In this review, we discuss the development pipeline for transcriptional biomarkers in molecular diagnostics and stress the importance of a reliable gene transcript quantification strategy. Hence, a further focus is put on the MIQE guidelines and how to adapt them for biomarker discovery, from signat...

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Detalles Bibliográficos
Autores principales: Grätz, Christian, Bui, Maria L. U., Thaqi, Granit, Kirchner, Benedikt, Loewe, Robert P., Pfaffl, Michael W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953338/
https://www.ncbi.nlm.nih.gov/pubmed/35330136
http://dx.doi.org/10.3390/life12030386

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953338/
https://www.ncbi.nlm.nih.gov/pubmed/35330136
http://dx.doi.org/10.3390/life12030386

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