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Obtaining Reliable RT-qPCR Results in Molecular Diagnostics—MIQE Goals and Pitfalls for Transcriptional Biomarker Discovery
In this review, we discuss the development pipeline for transcriptional biomarkers in molecular diagnostics and stress the importance of a reliable gene transcript quantification strategy. Hence, a further focus is put on the MIQE guidelines and how to adapt them for biomarker discovery, from signat...
Autores principales: | Grätz, Christian, Bui, Maria L. U., Thaqi, Granit, Kirchner, Benedikt, Loewe, Robert P., Pfaffl, Michael W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953338/ https://www.ncbi.nlm.nih.gov/pubmed/35330136 http://dx.doi.org/10.3390/life12030386 |
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