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Development of an Event-Specific Droplet Digital PCR Assay for Quantification and Evaluation of the Transgene DNAs in Trace Samples of GM PRNP-Knockout Goat

The prion protein (PRNP) gene encoding prion protein is considered a prerequisite for the occurrence of scrapie disease, and knockout of the PRNP gene in transgenic goat is one effective approach to avoid scrapie. This study aims to establish an event-specific droplet digital polymerase chain reacti...

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Detalles Bibliográficos
Autores principales: Xu, Wenting, Shen, Ping, Li, Rong, Liu, Biao, Yang, Litao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953510/
https://www.ncbi.nlm.nih.gov/pubmed/35327291
http://dx.doi.org/10.3390/foods11060868