Sensitive UHPLC-MS/MS quantification method for 4β- and 4α-hydroxycholesterol in plasma for accurate CYP3A phenotyping

4β-Hydroxycholesterol (4β-OHC) is formed by Cytochrome P450 (CYP)3A and has drawn attention as an endogenous phenotyping probe for CYP3A activity. However, 4β-OHC is also increased by cholesterol autooxidation occurring in vitro due to dysregulated storage and in vivo by oxidative stress or inflamma...

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Detalles Bibliográficos
Autores principales: Suzuki, Yosuke, Oda, Ayako, Negami, Jun, Toyama, Daiki, Tanaka, Ryota, Ono, Hiroyuki, Ando, Tadasuke, Shin, Toshitaka, Mimata, Hiromitsu, Itoh, Hiroki, Ohno, Keiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953653/
https://www.ncbi.nlm.nih.gov/pubmed/35181316
http://dx.doi.org/10.1016/j.jlr.2022.100184

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953653/
https://www.ncbi.nlm.nih.gov/pubmed/35181316
http://dx.doi.org/10.1016/j.jlr.2022.100184

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