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High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant Determination

For tiling of the SARS-CoV-2 genome, the ARTIC Network provided a V4 protocol using 99 pairs of primers for amplicon production and is currently the widely used amplicon-based approach. However, this technique has regions of low sequence coverage and is labour-, time-, and cost-intensive. Moreover,...

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Detalles Bibliográficos
Autores principales: Liao, Yu-Chieh, Chen, Feng-Jui, Chuang, Min-Chieh, Wu, Han-Chieh, Ji, Wan-Chen, Yu, Guann-Yi, Huang, Tsi-Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954144/
https://www.ncbi.nlm.nih.gov/pubmed/35328676
http://dx.doi.org/10.3390/ijms23063257