Antibodies to biotin enable large-scale detection of biotinylation sites on proteins

Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX pe...

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Detalles Bibliográficos
Autores principales: Udeshi, Namrata D, Pedram, Kayvon, Svinkina, Tanya, Fereshetian, Shaunt, Myers, Samuel A, Aygun, Ozan, Krug, Karsten, Clauser, Karl, Ryan, Dominic, Ast, Tslil, Mootha, Vamsi K, Ting, Alice Y, Carr, Steven A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954634/
https://www.ncbi.nlm.nih.gov/pubmed/29039416
http://dx.doi.org/10.1038/nmeth.4465

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954634/
https://www.ncbi.nlm.nih.gov/pubmed/29039416
http://dx.doi.org/10.1038/nmeth.4465

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