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Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing

SIMPLE SUMMARY: The iconic citrus crop of Florida is suffering from an incurable disease called Huanglongbing (HLB), or citrus greening. HLB is caused by a bacterium Candidatus Liberibacter asiaticus (CLas), which is efficiently vectored by a small insect, the Asian citrus psyllid (ACP). To control...

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Autores principales: Britt, Kellee, Gebben, Samantha, Levy, Amit, Achor, Diann, Sieburth, Peggy, Stevens, Kristian, Al Rwahnih, Maher, Batuman, Ozgur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954720/
https://www.ncbi.nlm.nih.gov/pubmed/35323573
http://dx.doi.org/10.3390/insects13030275
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author Britt, Kellee
Gebben, Samantha
Levy, Amit
Achor, Diann
Sieburth, Peggy
Stevens, Kristian
Al Rwahnih, Maher
Batuman, Ozgur
author_facet Britt, Kellee
Gebben, Samantha
Levy, Amit
Achor, Diann
Sieburth, Peggy
Stevens, Kristian
Al Rwahnih, Maher
Batuman, Ozgur
author_sort Britt, Kellee
collection PubMed
description SIMPLE SUMMARY: The iconic citrus crop of Florida is suffering from an incurable disease called Huanglongbing (HLB), or citrus greening. HLB is caused by a bacterium Candidatus Liberibacter asiaticus (CLas), which is efficiently vectored by a small insect, the Asian citrus psyllid (ACP). To control the spread of CLas and ultimately HLB, citrus growers primarily depend on environmentally harmful insecticides against the ACP, which can induce insecticide resistance. An alternative, biological approach involves insect-specific viruses (ISVs) of the ACP for future exploitation to exclusively target this insect pest. To identify these ISVs, we have subjected ACPs to high-throughput sequencing, a powerful sequencing technology. This current study follows an initial detection of citrus tristeza virus (CTV) amongst the viruses detected in the ACP during HTS and continues to show the persistent and diverse presence of CTV in Florida ACPs across subsequent years. We suggest that the ACP may be utilized as a tool or lens of CTV presence throughout Florida citrus groves. ABSTRACT: The destructive citrus disease, Huanglongbing (HLB) or citrus greening, continues to devastate Florida’s citrus industry. A hemipteran insect, the Asian citrus psyllid (ACP), disperses Candidatus Liberibacter asiaticus, one of the putative bacterial pathogens of HLB. This study builds upon ongoing research utilizing high-throughput sequencing to analyze the virome of ACP populations collected from citrus groves throughout Florida. Following the widespread detection of sequences aligning to the genome of citrus tristeza virus (CTV) across consecutive years in the Florida ACP virome, we continued to detect a pervasive amount of CTV in Florida ACPs during subsequent years. Simultaneously, we also detected mixed infections of CTV strains in pooled ACPs from different Florida regions. Predating the HLB epidemic, CTV has been present in Florida for many years and our results confirm its widespread and diverse persistence in Florida citrus groves through a unique lens, the ACP. CTV presence in the ACP likely results from feeding on CTV-infected citrus trees in Florida citrus groves, which may help to understand an overlapping presence of CTV and HLB, both endemic citrus pathosystems in the state, and their role in future integrated pest management strategies.
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spelling pubmed-89547202022-03-26 Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing Britt, Kellee Gebben, Samantha Levy, Amit Achor, Diann Sieburth, Peggy Stevens, Kristian Al Rwahnih, Maher Batuman, Ozgur Insects Article SIMPLE SUMMARY: The iconic citrus crop of Florida is suffering from an incurable disease called Huanglongbing (HLB), or citrus greening. HLB is caused by a bacterium Candidatus Liberibacter asiaticus (CLas), which is efficiently vectored by a small insect, the Asian citrus psyllid (ACP). To control the spread of CLas and ultimately HLB, citrus growers primarily depend on environmentally harmful insecticides against the ACP, which can induce insecticide resistance. An alternative, biological approach involves insect-specific viruses (ISVs) of the ACP for future exploitation to exclusively target this insect pest. To identify these ISVs, we have subjected ACPs to high-throughput sequencing, a powerful sequencing technology. This current study follows an initial detection of citrus tristeza virus (CTV) amongst the viruses detected in the ACP during HTS and continues to show the persistent and diverse presence of CTV in Florida ACPs across subsequent years. We suggest that the ACP may be utilized as a tool or lens of CTV presence throughout Florida citrus groves. ABSTRACT: The destructive citrus disease, Huanglongbing (HLB) or citrus greening, continues to devastate Florida’s citrus industry. A hemipteran insect, the Asian citrus psyllid (ACP), disperses Candidatus Liberibacter asiaticus, one of the putative bacterial pathogens of HLB. This study builds upon ongoing research utilizing high-throughput sequencing to analyze the virome of ACP populations collected from citrus groves throughout Florida. Following the widespread detection of sequences aligning to the genome of citrus tristeza virus (CTV) across consecutive years in the Florida ACP virome, we continued to detect a pervasive amount of CTV in Florida ACPs during subsequent years. Simultaneously, we also detected mixed infections of CTV strains in pooled ACPs from different Florida regions. Predating the HLB epidemic, CTV has been present in Florida for many years and our results confirm its widespread and diverse persistence in Florida citrus groves through a unique lens, the ACP. CTV presence in the ACP likely results from feeding on CTV-infected citrus trees in Florida citrus groves, which may help to understand an overlapping presence of CTV and HLB, both endemic citrus pathosystems in the state, and their role in future integrated pest management strategies. MDPI 2022-03-10 /pmc/articles/PMC8954720/ /pubmed/35323573 http://dx.doi.org/10.3390/insects13030275 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Britt, Kellee
Gebben, Samantha
Levy, Amit
Achor, Diann
Sieburth, Peggy
Stevens, Kristian
Al Rwahnih, Maher
Batuman, Ozgur
Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing
title Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing
title_full Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing
title_fullStr Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing
title_full_unstemmed Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing
title_short Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing
title_sort analysis of citrus tristeza virus incidences within asian citrus psyllid (diaphorina citri) populations in florida via high-throughput sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954720/
https://www.ncbi.nlm.nih.gov/pubmed/35323573
http://dx.doi.org/10.3390/insects13030275
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