Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences

CRISPR/Cas complexes enable precise gene editing in a wide variety of organisms. While the rigid identification of DNA sequences by these systems minimizes the potential for off-target effects, it consequently poses a problem for the recognition of sequences containing naturally occurring polymorphi...

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Detalles Bibliográficos
Autores principales: Krysler, Amanda R., Cromwell, Christopher R., Tu, Tommy, Jovel, Juan, Hubbard, Basil P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956631/
https://www.ncbi.nlm.nih.gov/pubmed/35338140
http://dx.doi.org/10.1038/s41467-022-29202-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956631/
https://www.ncbi.nlm.nih.gov/pubmed/35338140
http://dx.doi.org/10.1038/s41467-022-29202-x

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