NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens

The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a novel chassis organism for fundamental research and biotechnology. To fully harness the potential of this bacterium, highly efficient genome editing methods are indispensable to create strains tailored for spe...

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Detalles Bibliográficos
Autores principales: Stukenberg, Daniel, Hoff, Josef, Faber, Anna, Becker, Anke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956659/
https://www.ncbi.nlm.nih.gov/pubmed/35338236
http://dx.doi.org/10.1038/s42003-022-03150-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956659/
https://www.ncbi.nlm.nih.gov/pubmed/35338236
http://dx.doi.org/10.1038/s42003-022-03150-0

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