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NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens

The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a novel chassis organism for fundamental research and biotechnology. To fully harness the potential of this bacterium, highly efficient genome editing methods are indispensable to create strains tailored for spe...

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Detalles Bibliográficos
Autores principales:	Stukenberg, Daniel, Hoff, Josef, Faber, Anna, Becker, Anke
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956659/ https://www.ncbi.nlm.nih.gov/pubmed/35338236 http://dx.doi.org/10.1038/s42003-022-03150-0

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