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Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan

Cell-free system has emerged as a powerful platform with a wide range of in vitro applications and recently has contributed to express metabolic pathways for biosynthesis. Here we report in vitro construction of a native biosynthetic pathway for L-4-nitrotryptophan (L-4-nitro-Trp) synthesis using an...

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Detalles Bibliográficos
Autores principales: Tian, Xintong, Liu, Wan-Qiu, Xu, Huiling, Ji, Xiangyang, Liu, Yushi, Li, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956912/
https://www.ncbi.nlm.nih.gov/pubmed/35387232
http://dx.doi.org/10.1016/j.synbio.2022.03.006