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Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion
Acute kidney injury due to ischemia followed by reperfusion (IR) is a severe clinical condition with high death rates. IR affects the proximal tubule segments due to their predominantly oxidative metabolism and profoundly altered mitochondrial functions. We previously described the impact of IR on o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958683/ https://www.ncbi.nlm.nih.gov/pubmed/35343271 http://dx.doi.org/10.1177/09636897221085883 |
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author | Rodrigues-Ferreira, Clara Lopes, Jarlene Alécia Carneiro, Priscila Fonseca dos Santos Lessa, Cristiane Galina, Antonio Vieyra, Adalberto |
author_facet | Rodrigues-Ferreira, Clara Lopes, Jarlene Alécia Carneiro, Priscila Fonseca dos Santos Lessa, Cristiane Galina, Antonio Vieyra, Adalberto |
author_sort | Rodrigues-Ferreira, Clara |
collection | PubMed |
description | Acute kidney injury due to ischemia followed by reperfusion (IR) is a severe clinical condition with high death rates. IR affects the proximal tubule segments due to their predominantly oxidative metabolism and profoundly altered mitochondrial functions. We previously described the impact of IR on oxygen consumption, the generation of membrane potential (ΔΨ), and formation of reactive oxygen species, together with inflammatory and structural alterations. We also demonstrated the benefits of bone marrow mononuclear cells (BMMC) administration in these alterations. The objective of the present study has been to investigate the effect of IR and the influence of BMMC on the mechanisms of Ca(2+) handling in mitochondria of the proximal tubule cells. IR inhibited the rapid accumulation of Ca(2+) (Ca(2+) green fluorescence assays) and induced the opening of the cyclosporine A-sensitive permeability transition pore (PTP), alterations prevented by BMMC. IR accelerated Ca(2+)-induced decrease of ΔΨ (Safranin O fluorescence assays), as evidenced by decreased requirement for Ca(2+) load and t(1/2) for complete depolarization. Addition of BMMC and ADP recovered the normal depolarization profile, suggesting that stabilization of the adenine nucleotide translocase (ANT) in a conformation that inhibits PTP opening offers a partial defense mechanism against IR injury. Moreover, as ANT forms a complex with the voltage-dependent anion channel (VDAC) in the outer mitochondrial membrane, it is possible that this complex is also a target for IR injury—thus favoring Ca(2+) release, as well as the supramolecular structure that BMMC protects. These beneficial effects are accompanied by a stimulus of the citric acid cycle—which feed the mitochondrial complexes with the electrons removed from different substrates—as the result of accentuated stimulus of citrate synthase activity by BMMC. |
format | Online Article Text |
id | pubmed-8958683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-89586832022-03-29 Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion Rodrigues-Ferreira, Clara Lopes, Jarlene Alécia Carneiro, Priscila Fonseca dos Santos Lessa, Cristiane Galina, Antonio Vieyra, Adalberto Cell Transplant Original Article Acute kidney injury due to ischemia followed by reperfusion (IR) is a severe clinical condition with high death rates. IR affects the proximal tubule segments due to their predominantly oxidative metabolism and profoundly altered mitochondrial functions. We previously described the impact of IR on oxygen consumption, the generation of membrane potential (ΔΨ), and formation of reactive oxygen species, together with inflammatory and structural alterations. We also demonstrated the benefits of bone marrow mononuclear cells (BMMC) administration in these alterations. The objective of the present study has been to investigate the effect of IR and the influence of BMMC on the mechanisms of Ca(2+) handling in mitochondria of the proximal tubule cells. IR inhibited the rapid accumulation of Ca(2+) (Ca(2+) green fluorescence assays) and induced the opening of the cyclosporine A-sensitive permeability transition pore (PTP), alterations prevented by BMMC. IR accelerated Ca(2+)-induced decrease of ΔΨ (Safranin O fluorescence assays), as evidenced by decreased requirement for Ca(2+) load and t(1/2) for complete depolarization. Addition of BMMC and ADP recovered the normal depolarization profile, suggesting that stabilization of the adenine nucleotide translocase (ANT) in a conformation that inhibits PTP opening offers a partial defense mechanism against IR injury. Moreover, as ANT forms a complex with the voltage-dependent anion channel (VDAC) in the outer mitochondrial membrane, it is possible that this complex is also a target for IR injury—thus favoring Ca(2+) release, as well as the supramolecular structure that BMMC protects. These beneficial effects are accompanied by a stimulus of the citric acid cycle—which feed the mitochondrial complexes with the electrons removed from different substrates—as the result of accentuated stimulus of citrate synthase activity by BMMC. SAGE Publications 2022-03-26 /pmc/articles/PMC8958683/ /pubmed/35343271 http://dx.doi.org/10.1177/09636897221085883 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Rodrigues-Ferreira, Clara Lopes, Jarlene Alécia Carneiro, Priscila Fonseca dos Santos Lessa, Cristiane Galina, Antonio Vieyra, Adalberto Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion |
title | Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion |
title_full | Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion |
title_fullStr | Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion |
title_full_unstemmed | Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion |
title_short | Bone Marrow Mononuclear Cells Restore Normal Mitochondrial Ca(2+) Handling and Ca(2+)-Induced Depolarization of the Internal Mitochondrial Membrane by Inhibiting the Permeability Transition Pore After Ischemia/Reperfusion |
title_sort | bone marrow mononuclear cells restore normal mitochondrial ca(2+) handling and ca(2+)-induced depolarization of the internal mitochondrial membrane by inhibiting the permeability transition pore after ischemia/reperfusion |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958683/ https://www.ncbi.nlm.nih.gov/pubmed/35343271 http://dx.doi.org/10.1177/09636897221085883 |
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