Directed Evolution of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase Generates a Hyperactive and Highly Selective Variant

Pyrrolysyl-tRNA synthetase (PylRS) is frequently used for site-specific incorporation of noncanonical amino acids (ncAAs) into proteins. Recently, the active site of Methanomethylophilus alvus PylRS (MaPylRS) has been rationally engineered to expand its substrate compatibility, enabling the incorpor...

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Detalles Bibliográficos
Autores principales: Fischer, Jonathan T., Söll, Dieter, Tharp, Jeffery M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965510/
https://www.ncbi.nlm.nih.gov/pubmed/35372501
http://dx.doi.org/10.3389/fmolb.2022.850613

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965510/
https://www.ncbi.nlm.nih.gov/pubmed/35372501
http://dx.doi.org/10.3389/fmolb.2022.850613

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