Purification of synchronized Escherichia coli transcription elongation complexes by reversible immobilization on magnetic beads

Synchronized transcription elongation complexes (TECs) are a fundamental tool for in vitro studies of transcription and RNA folding. Transcription elongation can be synchronized by omitting one or more nucleoside triphosphates from an in vitro transcription reaction so that RNA polymerase can only t...

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Detalles Bibliográficos
Autores principales: Kelly, Skyler L., Szyjka, Courtney E., Strobel, Eric J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969151/
https://www.ncbi.nlm.nih.gov/pubmed/35247385
http://dx.doi.org/10.1016/j.jbc.2022.101789

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969151/
https://www.ncbi.nlm.nih.gov/pubmed/35247385
http://dx.doi.org/10.1016/j.jbc.2022.101789

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