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Impact of gene annotation choice on the quantification of RNA-seq data

BACKGROUND: RNA sequencing is currently the method of choice for genome-wide profiling of gene expression. A popular approach to quantify expression levels of genes from RNA-seq data is to map reads to a reference genome and then count mapped reads to each gene. Gene annotation data, which include c...

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Detalles Bibliográficos
Autores principales: Chisanga, David, Liao, Yang, Shi, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969366/
https://www.ncbi.nlm.nih.gov/pubmed/35354358
http://dx.doi.org/10.1186/s12859-022-04644-8