Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells

Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells usin...

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Autores principales: Wang, Tongjie, Lv, Cui, Hu, Fangxiao, Liu, Lijuan, Wang, Jinyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974948/
https://www.ncbi.nlm.nih.gov/pubmed/35402823
http://dx.doi.org/10.1097/BS9.0000000000000049
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author Wang, Tongjie
Lv, Cui
Hu, Fangxiao
Liu, Lijuan
Wang, Jinyong
author_facet Wang, Tongjie
Lv, Cui
Hu, Fangxiao
Liu, Lijuan
Wang, Jinyong
author_sort Wang, Tongjie
collection PubMed
description Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells using an inducible Runx1-Hoxa9-PSC (iR9-PSCs) line. The procedure mainly includes generation of induced hematopoietic progenitor cells (iHPCs) in vitro, transplantation, and development of functional induced T cells (iT) in vivo via transplantation. The entire induction process in vitro requires 21 days before iHPCs transplantation. The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation. We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose.
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spelling pubmed-89749482022-04-07 Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells Wang, Tongjie Lv, Cui Hu, Fangxiao Liu, Lijuan Wang, Jinyong Blood Sci Research Article Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells using an inducible Runx1-Hoxa9-PSC (iR9-PSCs) line. The procedure mainly includes generation of induced hematopoietic progenitor cells (iHPCs) in vitro, transplantation, and development of functional induced T cells (iT) in vivo via transplantation. The entire induction process in vitro requires 21 days before iHPCs transplantation. The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation. We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose. Wolters Kluwer Health 2020-07-25 /pmc/articles/PMC8974948/ /pubmed/35402823 http://dx.doi.org/10.1097/BS9.0000000000000049 Text en Copyright © 2020 The Authors. Published by Wolters Kluwer Health Inc., on behalf of the Chinese Association for Blood Sciences. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Research Article
Wang, Tongjie
Lv, Cui
Hu, Fangxiao
Liu, Lijuan
Wang, Jinyong
Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells
title Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells
title_full Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells
title_fullStr Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells
title_full_unstemmed Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells
title_short Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells
title_sort two-step protocol for regeneration of immunocompetent t cells from mouse pluripotent stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974948/
https://www.ncbi.nlm.nih.gov/pubmed/35402823
http://dx.doi.org/10.1097/BS9.0000000000000049
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