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Evaluation of Jumonji C lysine demethylase substrate preference to guide identification of in vitro substrates

Within the realm of lysine methylation, the discovery of lysine methyltransferase (KMTs) substrates has been burgeoning because of established systematic substrate screening protocols. Here, we describe a protocol enabling the systematic identification of JmjC KDM substrate preference and in vitro s...

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Detalles Bibliográficos
Autores principales: Hoekstra, Matthew, Chopra, Anand, Willmore, William G., Biggar, Kyle K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976124/
https://www.ncbi.nlm.nih.gov/pubmed/35378885
http://dx.doi.org/10.1016/j.xpro.2022.101271