The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs). However, it is still challenging to maintain high productivity...

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Autores principales: Liu, Hui-Ning, Dong, Wei-Hua, Lin, Yan, Zhang, Zhao-Hui, Wang, Tian-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977551/
https://www.ncbi.nlm.nih.gov/pubmed/35387297
http://dx.doi.org/10.3389/fbioe.2022.832065
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author Liu, Hui-Ning
Dong, Wei-Hua
Lin, Yan
Zhang, Zhao-Hui
Wang, Tian-Yun
author_facet Liu, Hui-Ning
Dong, Wei-Hua
Lin, Yan
Zhang, Zhao-Hui
Wang, Tian-Yun
author_sort Liu, Hui-Ning
collection PubMed
description Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs). However, it is still challenging to maintain high productivity ensuring the good quality of RTPs produced by CHO cells. MicroRNAs(miRNAs) are short regulatory non-coding RNAs that can regulate cellular behavior and complex phenotypes. It has been found that miRNAs can enhance the expression level of recombinant proteins in CHO cells by promoting proliferation, resisting apoptosis, and regulating metabolism. miRNAs also can affect the quality of RTPs. In this review, we will discuss the effect and mechanism of miRNA on the expression level and quality of recombinant proteins in CHO cells.
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spelling pubmed-89775512022-04-05 The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism Liu, Hui-Ning Dong, Wei-Hua Lin, Yan Zhang, Zhao-Hui Wang, Tian-Yun Front Bioeng Biotechnol Bioengineering and Biotechnology Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs). However, it is still challenging to maintain high productivity ensuring the good quality of RTPs produced by CHO cells. MicroRNAs(miRNAs) are short regulatory non-coding RNAs that can regulate cellular behavior and complex phenotypes. It has been found that miRNAs can enhance the expression level of recombinant proteins in CHO cells by promoting proliferation, resisting apoptosis, and regulating metabolism. miRNAs also can affect the quality of RTPs. In this review, we will discuss the effect and mechanism of miRNA on the expression level and quality of recombinant proteins in CHO cells. Frontiers Media S.A. 2022-03-21 /pmc/articles/PMC8977551/ /pubmed/35387297 http://dx.doi.org/10.3389/fbioe.2022.832065 Text en Copyright © 2022 Liu, Dong, Lin, Zhang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liu, Hui-Ning
Dong, Wei-Hua
Lin, Yan
Zhang, Zhao-Hui
Wang, Tian-Yun
The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
title The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
title_full The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
title_fullStr The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
title_full_unstemmed The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
title_short The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
title_sort effect of microrna on the production of recombinant protein in cho cells and its mechanism
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977551/
https://www.ncbi.nlm.nih.gov/pubmed/35387297
http://dx.doi.org/10.3389/fbioe.2022.832065
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