Cargando…

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs). However, it is still challenging to maintain high productivity...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Hui-Ning, Dong, Wei-Hua, Lin, Yan, Zhang, Zhao-Hui, Wang, Tian-Yun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977551/ https://www.ncbi.nlm.nih.gov/pubmed/35387297 http://dx.doi.org/10.3389/fbioe.2022.832065

Ejemplares similares

Decoupling Growth and Protein Production in CHO Cells: A Targeted Approach
por: Donaldson, James S., et al.
Publicado: (2021)

Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells
por: Wang, Xiao-yin, et al.
Publicado: (2022)

Engineering of CHO cells for the production of vertebrate recombinant sialyltransferases
por: Houeix, Benoit, et al.
Publicado: (2019)

MicroRNA-loaded biomaterials for osteogenesis
por: Wang, Jingwei, et al.
Publicado: (2022)

Bringing MicroRNAs to Light: Methods for MicroRNA Quantification and Visualization in Live Cells
por: Siddika, Tarana, et al.
Publicado: (2021)

Stabilizing and Anti-Repressor Elements Effectively Increases Transgene Expression in Transfected CHO Cells
por: Li, Qin, et al.
Publicado: (2022)

Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications
por: Hertel, Oliver, et al.
Publicado: (2022)

Omics Analysis Reveals the Mechanism of Enhanced Recombinant Protein Production Under Simulated Microgravity
por: Huangfu, Jie, et al.
Publicado: (2020)

Engineering protein glycosylation in CHO cells to be highly similar to murine host cells
por: Gupta, Shivani, et al.
Publicado: (2023)

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells
por: Wei, Mian, et al.
Publicado: (2022)

Enhancing and stabilizing monoclonal antibody production by Chinese hamster ovary (CHO) cells with optimized perfusion culture strategies
por: Liang, Kexue, et al.
Publicado: (2023)

MicroRNA Mediated Cardioprotection – Is There a Path to Clinical Translation?
por: Nazari-Shafti, Timo Z., et al.
Publicado: (2020)

Growth and eGFP Production of CHO-K1 Suspension Cells Cultivated From Single Cell to Laboratory Scale
por: Schmitz, Julian, et al.
Publicado: (2021)

Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO Cells
por: Mayrhofer, Patrick, et al.
Publicado: (2021)

The Serum MicroRNA Signatures for Pancreatic Cancer Detection and Operability Evaluation
por: Yan, Qiuliang, et al.
Publicado: (2020)

Highly efficient and automated isolation technology for extracellular vesicles microRNA
por: Di, Kaili, et al.
Publicado: (2022)

Proteomic Profiling of IgG1 Producing CHO Cells Using LC/LC-SPS-MS(3): The Effects of Bioprocessing Conditions on Productivity and Product Quality
por: Strasser, Lisa, et al.
Publicado: (2021)

Escherichia coli Can Adapt Its Protein Translocation Machinery for Enhanced Periplasmic Recombinant Protein Production
por: Karyolaimos, Alexandros, et al.
Publicado: (2020)

Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System
por: Park, Yu-Jin, et al.
Publicado: (2021)

MicroRNA-Mediated Direct Reprogramming of Human Adult Fibroblasts Toward Cardiac Phenotype
por: Paoletti, C., et al.
Publicado: (2020)

MicroRNAs in extracellular vesicles: Sorting mechanisms, diagnostic value, isolation, and detection technology
por: Xu, Dongjie, et al.
Publicado: (2022)

Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
por: Nguyen, Melanie, et al.
Publicado: (2023)

Production of Melanins With Recombinant Microorganisms
por: Martínez, Luz María, et al.
Publicado: (2019)

MicroRNA-146a Regulates Perfusion Recovery in Response to Arterial Occlusion via Arteriogenesis
por: Heuslein, Joshua L., et al.
Publicado: (2018)

Endothelial Cell Morphology Regulates Inflammatory Cells Through MicroRNA Transferred by Extracellular Vesicles
por: Liang, Jiaqi, et al.
Publicado: (2020)

Thermus thermophilus Argonaute-based signal amplifier for highly sensitive and specific microRNA detection
por: Wang, Ziqi, et al.
Publicado: (2023)

Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p
por: Liu, Kun, et al.
Publicado: (2022)

Model-based intensification of CHO cell cultures: One-step strategy from fed-batch to perfusion
por: Richelle, Anne, et al.
Publicado: (2022)

Selection of biophysically favorable antibody variants using a modified Flp-In CHO mammalian display platform
por: Huhtinen, Olli, et al.
Publicado: (2023)

Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production
por: Manfrão-Netto, João Heitor Colombelli, et al.
Publicado: (2019)

Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis
por: Cirkovic, Andja, et al.
Publicado: (2021)

Screening for MicroRNA combination with engineered exosomes as a new tool against osteosarcoma in elderly patients
por: Han, Jiyu, et al.
Publicado: (2022)

Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
por: Li, Weifeng, et al.
Publicado: (2021)

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
por: Karyolaimos, Alexandros, et al.
Publicado: (2021)

Photothermal Detection of MicroRNA Using a Horseradish Peroxidase-Encapsulated DNA Hydrogel With a Portable Thermometer
por: Liu, Xiujuan, et al.
Publicado: (2021)

Dynamic Modeling of CHO Cell Metabolism Using the Hybrid Cybernetic Approach With a Novel Elementary Mode Analysis Strategy
por: Martínez, Juan A., et al.
Publicado: (2020)

Recombinant β-Carotene Production by Yarrowia lipolytica – Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
por: Jacobsen, Irene Hjorth, et al.
Publicado: (2020)

Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells
por: Li, Zheng-Mei, et al.
Publicado: (2022)

microRNAs delivered by small extracellular vesicles in MSCs as an emerging tool for bone regeneration
por: Liu, Runyuan, et al.
Publicado: (2023)

A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality
por: Krebs, Simon K., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_1917hd1f2ekdv2jjpsv2t90b5p