Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practi...

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Detalles Bibliográficos
Autores principales: Zhao, Rujian, Yu, Chunxu, Lu, Baiyang, Li, Bingling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977996/
https://www.ncbi.nlm.nih.gov/pubmed/35425009
http://dx.doi.org/10.1039/d2ra01332k

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977996/
https://www.ncbi.nlm.nih.gov/pubmed/35425009
http://dx.doi.org/10.1039/d2ra01332k

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