Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practi...

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Detalles Bibliográficos
Autores principales: Zhao, Rujian, Yu, Chunxu, Lu, Baiyang, Li, Bingling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977996/
https://www.ncbi.nlm.nih.gov/pubmed/35425009
http://dx.doi.org/10.1039/d2ra01332k
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author Zhao, Rujian
Yu, Chunxu
Lu, Baiyang
Li, Bingling
author_facet Zhao, Rujian
Yu, Chunxu
Lu, Baiyang
Li, Bingling
author_sort Zhao, Rujian
collection PubMed
description We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum.
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spelling pubmed-89779962022-04-13 Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection Zhao, Rujian Yu, Chunxu Lu, Baiyang Li, Bingling RSC Adv Chemistry We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum. The Royal Society of Chemistry 2022-04-04 /pmc/articles/PMC8977996/ /pubmed/35425009 http://dx.doi.org/10.1039/d2ra01332k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhao, Rujian
Yu, Chunxu
Lu, Baiyang
Li, Bingling
Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection
title Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection
title_full Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection
title_fullStr Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection
title_full_unstemmed Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection
title_short Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection
title_sort coupling nucleic acid circuitry with the crispr-cas12a system for universal and signal-on detection
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977996/
https://www.ncbi.nlm.nih.gov/pubmed/35425009
http://dx.doi.org/10.1039/d2ra01332k
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AT lubaiyang couplingnucleicacidcircuitrywiththecrisprcas12asystemforuniversalandsignalondetection
AT libingling couplingnucleicacidcircuitrywiththecrisprcas12asystemforuniversalandsignalondetection

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