Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System

The nuclease activity of the CRISPR-Cas9 system relies on the delivery of a CRISPR-associated protein 9 (Cas9) and a single guide RNA (sgRNA) against the target gene. CRISPR components are typically delivered to cells as either a Cas9/sgRNA ribonucleoprotein (RNP) complex or a plasmid encoding a Cas...

Descripción completa

Detalles Bibliográficos
Autores principales: Shalaby, Karim E., Aouida, Mustapha, Gupta, Vijay, Ghanem, Simona S., El-Agnaf, Omar M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978543/
https://www.ncbi.nlm.nih.gov/pubmed/35386234
http://dx.doi.org/10.3389/fgeed.2022.854866

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978543/
https://www.ncbi.nlm.nih.gov/pubmed/35386234
http://dx.doi.org/10.3389/fgeed.2022.854866

Ejemplares similares