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Temperature non-uniformity detection on dPCR chips and temperature sensor calibration

A microfluidic-based digital polymerase chain reaction (dPCR) chip requires precise temperature control as well as uniform temperature distribution to ensure PCR efficiency. However, measuring local temperature and its distribution over thousands of μL/nL-volume samples with minimum disturbance is c...

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Detalles Bibliográficos
Autores principales: Gaňová, Martina, Wang, Xinlu, Yan, Zhiqiang, Zhang, Haoqing, Lednický, Tomáš, Korabečná, Marie, Neužil, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979175/
https://www.ncbi.nlm.nih.gov/pubmed/35425215
http://dx.doi.org/10.1039/d1ra08138a