Reconstitution of purified membrane protein dimers in lipid nanodiscs with defined stoichiometry and orientation using a split GFP tether

Many membrane proteins function as dimers or larger oligomers, including transporters, channels, certain signaling receptors, and adhesion molecules. In some cases, the interactions between individual proteins may be weak and/or dependent on specific lipids, such that detergent solubilization used f...

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Detalles Bibliográficos
Autores principales: Bruguera, Elise S., Mahoney, Jacob P., Weis, William I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980801/
https://www.ncbi.nlm.nih.gov/pubmed/35074428
http://dx.doi.org/10.1016/j.jbc.2022.101628

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980801/
https://www.ncbi.nlm.nih.gov/pubmed/35074428
http://dx.doi.org/10.1016/j.jbc.2022.101628

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