Detection of thermal shift in cellular Keap1 by protein-protein interaction inhibitors using immunoblot- and fluorescence microplate-based assays

Pharmacologic inhibition of the protein-protein interaction (PPI) interface of the Keap1:Nrf2 complex, which leads to Nrf2 activation and cytoprotective gene expression, offers a promising strategy for disease prevention and treatment. To facilitate identification and validation of small-molecule Ke...

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Detalles Bibliográficos
Autores principales: Dayalan Naidu, Sharadha, Dikovskaya, Dina, Moore, Terry W., Dinkova-Kostova, Albena T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980889/
https://www.ncbi.nlm.nih.gov/pubmed/35391936
http://dx.doi.org/10.1016/j.xpro.2022.101265

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980889/
https://www.ncbi.nlm.nih.gov/pubmed/35391936
http://dx.doi.org/10.1016/j.xpro.2022.101265

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