PNA microprobe for label-free detection of expanded trinucleotide repeats

We present a PNA microprobe sensing platform to detect trinucleotide repeat mutation by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington's disease-associated CAG repeats in cell-derived total RNA with S/N 1 : 3. This sensitive, label-free, and PCR-free de...

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Detalles Bibliográficos
Autores principales: Asefifeyzabadi, Narges, Durocher, Grace, Tshilenge, Kizito-Tshitoko, Alam, Tanimul, Ellerby, Lisa M., Shamsi, Mohtashim H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982460/
https://www.ncbi.nlm.nih.gov/pubmed/35424746
http://dx.doi.org/10.1039/d2ra00230b
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author Asefifeyzabadi, Narges
Durocher, Grace
Tshilenge, Kizito-Tshitoko
Alam, Tanimul
Ellerby, Lisa M.
Shamsi, Mohtashim H.
author_facet Asefifeyzabadi, Narges
Durocher, Grace
Tshilenge, Kizito-Tshitoko
Alam, Tanimul
Ellerby, Lisa M.
Shamsi, Mohtashim H.
author_sort Asefifeyzabadi, Narges
collection PubMed
description We present a PNA microprobe sensing platform to detect trinucleotide repeat mutation by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington's disease-associated CAG repeats in cell-derived total RNA with S/N 1 : 3. This sensitive, label-free, and PCR-free detection strategy may be employed in the future to develop biosensing platforms for the detection of a plethora of repeat expansion disorders.
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spelling pubmed-89824602022-04-13 PNA microprobe for label-free detection of expanded trinucleotide repeats Asefifeyzabadi, Narges Durocher, Grace Tshilenge, Kizito-Tshitoko Alam, Tanimul Ellerby, Lisa M. Shamsi, Mohtashim H. RSC Adv Chemistry We present a PNA microprobe sensing platform to detect trinucleotide repeat mutation by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington's disease-associated CAG repeats in cell-derived total RNA with S/N 1 : 3. This sensitive, label-free, and PCR-free detection strategy may be employed in the future to develop biosensing platforms for the detection of a plethora of repeat expansion disorders. The Royal Society of Chemistry 2022-03-10 /pmc/articles/PMC8982460/ /pubmed/35424746 http://dx.doi.org/10.1039/d2ra00230b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Asefifeyzabadi, Narges
Durocher, Grace
Tshilenge, Kizito-Tshitoko
Alam, Tanimul
Ellerby, Lisa M.
Shamsi, Mohtashim H.
PNA microprobe for label-free detection of expanded trinucleotide repeats
title PNA microprobe for label-free detection of expanded trinucleotide repeats
title_full PNA microprobe for label-free detection of expanded trinucleotide repeats
title_fullStr PNA microprobe for label-free detection of expanded trinucleotide repeats
title_full_unstemmed PNA microprobe for label-free detection of expanded trinucleotide repeats
title_short PNA microprobe for label-free detection of expanded trinucleotide repeats
title_sort pna microprobe for label-free detection of expanded trinucleotide repeats
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982460/
https://www.ncbi.nlm.nih.gov/pubmed/35424746
http://dx.doi.org/10.1039/d2ra00230b
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