An optimized approach for processing of frozen lung and lavage samples for microbiome studies

The respiratory tract has a resident microbiome with low biomass and limited diversity. This results in difficulties with sample preparation for sequencing due to uneven bacteria-to-host DNA ratio, especially for small tissue samples such as mouse lungs. We compared effectiveness of current procedur...

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Detalles Bibliográficos
Autores principales: Wiscovitch-Russo, Rosana, Singh, Harinder, Oldfield, Lauren M., Fedulov, Alexey V., Gonzalez-Juarbe, Norberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982836/
https://www.ncbi.nlm.nih.gov/pubmed/35381030
http://dx.doi.org/10.1371/journal.pone.0265891

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982836/
https://www.ncbi.nlm.nih.gov/pubmed/35381030
http://dx.doi.org/10.1371/journal.pone.0265891

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