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Agonist and antagonist TRUPATH assays for G protein-coupled receptors

TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors. Here, we present protocols for agonist and antagonist TRUPATH assays in the 384-well plate format, thereby providing an o...

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Detalles Bibliográficos
Autores principales: DiBerto, Jeffrey F., Smart, Katie, Olsen, Reid H.J., Roth, Bryan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983427/
https://www.ncbi.nlm.nih.gov/pubmed/35403009
http://dx.doi.org/10.1016/j.xpro.2022.101259