Agonist and antagonist TRUPATH assays for G protein-coupled receptors

TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors. Here, we present protocols for agonist and antagonist TRUPATH assays in the 384-well plate format, thereby providing an o...

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Detalles Bibliográficos
Autores principales: DiBerto, Jeffrey F., Smart, Katie, Olsen, Reid H.J., Roth, Bryan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983427/
https://www.ncbi.nlm.nih.gov/pubmed/35403009
http://dx.doi.org/10.1016/j.xpro.2022.101259
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author DiBerto, Jeffrey F.
Smart, Katie
Olsen, Reid H.J.
Roth, Bryan L.
author_facet DiBerto, Jeffrey F.
Smart, Katie
Olsen, Reid H.J.
Roth, Bryan L.
author_sort DiBerto, Jeffrey F.
collection PubMed
description TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors. Here, we present protocols for agonist and antagonist TRUPATH assays in the 384-well plate format, thereby providing an opportunity for higher throughput. We also provide both data analysis and quality control analyses for these assays, along with considerations for assay optimization and solutions for troubleshooting needs that may be encountered. For complete details on the use and execution of this protocol, please refer to Olsen et al. (2020).
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spelling pubmed-89834272022-04-07 Agonist and antagonist TRUPATH assays for G protein-coupled receptors DiBerto, Jeffrey F. Smart, Katie Olsen, Reid H.J. Roth, Bryan L. STAR Protoc Protocol TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors. Here, we present protocols for agonist and antagonist TRUPATH assays in the 384-well plate format, thereby providing an opportunity for higher throughput. We also provide both data analysis and quality control analyses for these assays, along with considerations for assay optimization and solutions for troubleshooting needs that may be encountered. For complete details on the use and execution of this protocol, please refer to Olsen et al. (2020). Elsevier 2022-04-01 /pmc/articles/PMC8983427/ /pubmed/35403009 http://dx.doi.org/10.1016/j.xpro.2022.101259 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
DiBerto, Jeffrey F.
Smart, Katie
Olsen, Reid H.J.
Roth, Bryan L.
Agonist and antagonist TRUPATH assays for G protein-coupled receptors
title Agonist and antagonist TRUPATH assays for G protein-coupled receptors
title_full Agonist and antagonist TRUPATH assays for G protein-coupled receptors
title_fullStr Agonist and antagonist TRUPATH assays for G protein-coupled receptors
title_full_unstemmed Agonist and antagonist TRUPATH assays for G protein-coupled receptors
title_short Agonist and antagonist TRUPATH assays for G protein-coupled receptors
title_sort agonist and antagonist trupath assays for g protein-coupled receptors
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983427/
https://www.ncbi.nlm.nih.gov/pubmed/35403009
http://dx.doi.org/10.1016/j.xpro.2022.101259
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