Cargando…

Agonist and antagonist TRUPATH assays for G protein-coupled receptors

TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors. Here, we present protocols for agonist and antagonist TRUPATH assays in the 384-well plate format, thereby providing an o...

Descripción completa

Detalles Bibliográficos
Autores principales:	DiBerto, Jeffrey F., Smart, Katie, Olsen, Reid H.J., Roth, Bryan L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983427/ https://www.ncbi.nlm.nih.gov/pubmed/35403009 http://dx.doi.org/10.1016/j.xpro.2022.101259

Ejemplares similares

“TRUPATH, an Open-Source Biosensor Platform for Interrogating the GPCR Transducerome”
por: Olsen, Reid H.J., et al.
Publicado: (2020)

De Novo Design of κ-Opioid Receptor Antagonists Using a Generative Deep-Learning Framework
por: Salas-Estrada, Leslie, et al.
Publicado: (2023)

A machine learning model for classifying G-protein-coupled receptors as agonists or antagonists
por: Oh, Jooseong, et al.
Publicado: (2022)

Function-based high-throughput screening for antibody antagonists and agonists against G protein-coupled receptors
por: Ren, Huanhuan, et al.
Publicado: (2020)

Dynamical Binding Modes Determine Agonistic and Antagonistic Ligand Effects in the Prostate-Specific G-Protein Coupled Receptor (PSGR)
por: Wolf, Steffen, et al.
Publicado: (2017)

DREADD Agonist 21 Is an Effective Agonist for Muscarinic-Based DREADDs in Vitro and in Vivo
por: Thompson, Karen J., et al.
Publicado: (2018)

Muscarinic Receptor Agonists and Antagonists
por: Broadley, Kenneth J., et al.
Publicado: (2001)

Protocol for identifying physiologically relevant binding proteins of G-protein-coupled receptors
por: Huang, Bill X., et al.
Publicado: (2023)

Biosynthesis of lanthionine-constrained agonists of G protein-coupled receptors
por: Moll, Gert N., et al.
Publicado: (2020)

Protocol to characterize G(i/o) and G(s) protein-coupled receptors in transiently transfected cells using ELISA and cAMP measurements
por: Liebing, Aenne-Dorothea, et al.
Publicado: (2023)

Protocol for evaluation and validation of TLR8 antagonists in HEK-Blue cells via secreted embryonic alkaline phosphatase assay
por: Hu, Zhenyi, et al.
Publicado: (2021)

Structural insights into the human niacin receptor HCA2-G(i) signalling complex
por: Yang, Yang, et al.
Publicado: (2023)

Chronic Activation of the G Protein-Coupled Receptor 30 with Agonist G-1 Attenuates Heart Failure
por: Kang, Shoulei, et al.
Publicado: (2012)

A Pharmacological Organization of G Protein-coupled Receptors
por: Lin, Henry, et al.
Publicado: (2013)

C. elegans survival assays to discern global and transcription-coupled nucleotide excision repair
por: van der Woude, Melanie, et al.
Publicado: (2021)

G protein-coupled receptor inactivation by an allosteric inverse-agonist antibody
por: Hino, Tomoya, et al.
Publicado: (2012)

Novel inverse agonists for the orphan G protein-coupled receptor 6
por: Laun, Alyssa S., et al.
Publicado: (2018)

Selective Photoswitchable Allosteric Agonist of a G Protein-Coupled Receptor
por: Donthamsetti, Prashant, et al.
Publicado: (2021)

Evidence for biased agonists and antagonists at the endothelin receptors
por: Maguire, Janet J.
Publicado: (2016)

Non-Peptide Agonists and Antagonists of the Prokineticin Receptors
por: Lattanzi, Roberta, et al.
Publicado: (2022)

Development of a FLIPR Assay for the Simultaneous Identification of MrgD Agonists and Antagonists from a Single Screen
por: Ajit, Seena K., et al.
Publicado: (2010)

A surface plasmon resonance-based method for monitoring interactions between G protein-coupled receptors and interacting proteins
por: Stroth, Nikolas
Publicado: (2016)

Restoration of bilateral motor coordination from preserved agonist-antagonist coupling in amputation musculature
por: Shu, Tony, et al.
Publicado: (2021)

Engineering of chimeric peptides as antagonists for the G protein-coupled receptor, RXFP4
por: Praveen, Praveen, et al.
Publicado: (2019)

Colloidal Aggregation Causes Inhibition of G Protein-Coupled Receptors
por: Sassano, Maria F., et al.
Publicado: (2013)

Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations
por: Gao, Yunrong, et al.
Publicado: (2021)

Single-chain fluorescent integrators for mapping G-protein-coupled receptor agonists
por: Kroning, Kayla, et al.
Publicado: (2023)

The Agonist-antagonist Myoneural Interface
por: Herr, Hugh, et al.
Publicado: (2021)

Engineered G-protein Coupled Receptors are Powerful Tools to Investigate Biological Processes and Behaviors
por: Nichols, Charles D., et al.
Publicado: (2009)

Nanobody-enabled monitoring of kappa opioid receptor states
por: Che, Tao, et al.
Publicado: (2020)

High-throughput screening of histone deacetylases and determination of kinetic parameters using fluorogenic assays
por: Moreno-Yruela, Carlos, et al.
Publicado: (2021)

Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay
por: Taylor, Amber W., et al.
Publicado: (2019)

Estrogen and G protein-coupled estrogen receptor agonist G-1 cause relaxation of human gallbladder
por: Lee, Ming-Che, et al.
Publicado: (2016)

Conformational dynamics of androgen receptors bound to agonists and antagonists
por: Gim, Hyo Jin, et al.
Publicado: (2021)

Correction to: Restoration of bilateral motor coordination from preserved agonist-antagonist coupling in amputation musculature
por: Shu, Tony, et al.
Publicado: (2021)

Changes in Sensitivity of Reward and Motor Behavior to Dopaminergic, Glutamatergic, and Cholinergic Drugs in a Mouse Model of Fragile X Syndrome
por: Fish, Eric W., et al.
Publicado: (2013)

Impact of G29179T mutation on two commercial PCR assays for SARS-CoV-2 detection
por: Kami, Wakaki, et al.
Publicado: (2023)

Hyodeoxycholic acid derivatives as liver X receptor α and G-protein-coupled bile acid receptor agonists
por: De Marino, Simona, et al.
Publicado: (2017)

Protocol for crystal structure determination of the antagonist-bound human cannabinoid receptor CB2
por: Shen, Ling, et al.
Publicado: (2021)

A G protein–coupled receptor and the intracellular synthase of its agonist functionally cooperate
por: Binda, Chantal, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_ju0cktcj01auffo11irganmqdp