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Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein

We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with increasing pressure. At atmospheric pressure, anisotropy de...

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Detalles Bibliográficos
Autores principales: Kaur, Harpreet, Nguyen, Khanh, Kumar, Pradeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8984833/
https://www.ncbi.nlm.nih.gov/pubmed/35424839
http://dx.doi.org/10.1039/d1ra08977c