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Direct measurements of mRNA translation kinetics in living cells

Ribosome mediated mRNA translation is central to life. The cycle of translation, however, has been characterized mostly using reconstituted systems, with only few techniques applicable for studies in the living cell. Here we describe a live-cell ribosome-labeling method, which allows us to character...

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Detalles Bibliográficos
Autores principales: Metelev, Mikhail, Lundin, Erik, Volkov, Ivan L., Gynnå, Arvid H., Elf, Johan, Johansson, Magnus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986856/
https://www.ncbi.nlm.nih.gov/pubmed/35388013
http://dx.doi.org/10.1038/s41467-022-29515-x