An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry

Membrane proteins (MPs) are essential in many cellular functions. To maintain proteostasis, MPs are downregulated via ubiquitination and degradation. Here, we describe an optimized protocol to analyze MP degradation using quantitative western blot and flow cytometry-based approaches. We use the degr...

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Detalles Bibliográficos
Autores principales: Arines, Felichi Mae, Li, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987394/
https://www.ncbi.nlm.nih.gov/pubmed/35403002
http://dx.doi.org/10.1016/j.xpro.2022.101274

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987394/
https://www.ncbi.nlm.nih.gov/pubmed/35403002
http://dx.doi.org/10.1016/j.xpro.2022.101274

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